Alnapharm
Alnapharm
Alnapharm
Alnapharm

Gene Cloning services

Gene Cloning services include Cloning and Sub-cloning. The expression vectors we use are optimized for high level expression. The Sub-cloning procedure also provides gene manipulation including tag fusion, cleavage site insertion. If you do not have a gene available, we can close the gene based on the provided accession number. Here is a break down of the cloning procedure that we offer:

  • Amplify your gene of interest from a cDNA library, genomic DNA or a Bac clone by PCR
  • Clone the PCR product into a cloning vector and then
  • Sub-clone the gene into our expression vector
  • The cloned gene will be sequenced in both orientations to ensure the quality

Gene Synthesis Services:

If your gene of interest is rare in nature or the genetic material containing your gene is hard to get, it could be difficult and costly to clone your gene-of-interest by gene cloning. In these cases we synthesize your gene-of-interest de novo. By synthesizing oligos overlapped each other at the ends, these oligos can be annealed and extended to form a full length gene.

Due to the nature of oligo synthesis,mutations, especially deletions are happened often in these synthesized genes. These dramatically limit the gene size and increase the cost of the gene synthesis. By a novel mutation removing technique, we can efficiently synthesize gene in large size.

Site-directed Mutagenesis:

Whether you want to manipulate your gene for expressing mutated proteins or other purposes, we can help you manipulate the gene by site directed insertion, deletion, or point mutagenesis.

Mission: Products for life

"Discover more science." Alna-Bioscience’s motto expresses the enterprise’s goal to stay connected with healthy life. It has committed itself to life science...

Contact

Alna-Bioscience GmbH & Co. KG
Poppenbütteler Bogen 68
D-22399 Hamburg – Germany
Phone: +49 (0)40 600 88 55-0
Fax: +49 (0)40 600 88 55-50
E-Mail:

Customized Services

Alna-Bioscience is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

more than 1000 products

One major vision guides all our highly specialized, tailor-made solutions. This is, as we consider, the ideal starting point for our common goal of laying more milestones in biotechnology while remaining in close cooperation with our clients. "Discover more science."