Recombinant Protein Expression

Using multiple expression systems, including bacterial, insect, and mammalian expression systems, Alna-Bioscience has the capability to produce hard to express protein at milligram level. Whether you are focused on lead discovery, assay development, vaccine production, high throughput screening, or characterization of interesting gene products, we can find a solution for you. We provide a wide range of choices for protein expression with high quality in bacterial, yeast, baculovirus/insect cell, and mammalian cell systems, at both small and large scales.

E. Coli Expression

Transformation of the expression vector into E.Coli
Screening and selection of positive clones
Evaluation of expression by SDS-PAGE or Western blotting
Optimization of host strain with small-scale analysis in different E.Coli backgrounds
Growth and expression optimization by varying induction, temperature, cell density, time, antibiotics, media to overcome low production and/or insoluble protein production
Inclusion body isolation and preparation
Cell harvesting and lvsate preparation

Baculovirus Expression

Transfection of insect cells and generation of recombinant virus
Viral production and plaque purification
Test expression of recombinant virus clones
Amplification and purification of recombinant baculovirus
Viral titer determination and generation of high-titer virus stock
Expression optimization is Sf9 or HiFive cells and varying temperature, MOI, growth conditions, media
Solubility analysis
Large-scale culture protein production/li>
Cell harvesting (or conditioned medium) and lvsate preparation
High throughput screening of your multiple plasmids to find the most desired construct
Producing high titer viruses in one week

Large-scale Fermentation

High density cell production in shaker flasks or bio-reactors
Continuous flow, high-speed centrifugation for cell-paste recovery
Low temperature, foam-free, high-pressure cell lysis system

Expression System Capabilities

System	Capabilities
Bacteria	Provides transformed bacterial paste for large-scale fermentation, up to three metric tons. Provides homogeneous recombinant proteins, over 98% pure, for crystallization, and partially purified recombinant proteins, over 70% pure. Provides large quantities of protein, up to 1 kg
Yeast	Provides yeast cell pellets transformed for large-scale fermentation, up to three metric tons. Provides homogeneous recombinant proteins, over 98% pure, for crystallization. Provides partially purified recombinant proteins, over 70% pure. Provides large quantities of protein, up to 1 kg.
Baculovirus-Insect Cells	Provides purified, high titer recombinant baculovirus stock, of up to 109 pfu/ml, carrying the customer's gene. Provides homogeneous recombinant proteins, over 98% pure, for crystallization. Provides partially purified recombinant proteins, over 70% pure. Provides large quantities of protein, up to 1 kg. Provides a large-scale protein production option, up to 500 L, using insect cells and a wave mixer system.
Mammaclian Cells	Provides one single system for the production of final homogeneous recombinant proteins, from gene synthesis to expression, for orders of up to 100 mg. Permits transient expression of customers' proteins of interest, up to 10 mg in 10 days. Provides conditioned media and cell pellets from stable cell lines, up to 300 L, using a wave mixer system.

Mission: Products for life

"Discover more science." Alna-Bioscience’s motto expresses the enterprise’s goal to stay connected with healthy life. It has committed itself to life science...

Contact

Alna-Bioscience GmbH & Co. KG
Poppenbütteler Bogen 68
D-22399 Hamburg – Germany
Phone: +49 (0)40 600 88 55-0
Fax: +49 (0)40 600 88 55-50
E-Mail: info@alna-bioscience.com

Customized Services

Alna-Bioscience is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

more than 1000 products

One major vision guides all our highly specialized, tailor-made solutions. This is, as we consider, the ideal starting point for our common goal of laying more milestones in biotechnology while remaining in close cooperation with our clients. "Discover more science."